Error Analysis for Protein Conformation Quantities in Circular Dichroism Spectrum

Circular Dichroism of Protein

Estimation of protein secondary structure and error analysis from circular dichroism spectra.

The estimation of protein secondary structure from circular dichroism spectra is described by a multivariate linear model with noise (Gauss-Markoff model). With this formalism the adequacy of the linear model is investigated, paying special attention to the estimation of the error in the secondary structure estimates. It is shown that the linear model is only adequate for the alpha-helix class....

Circular dichroism studies at variable temperature: urobilinoid conformation.

Measurements of the temperature dependence of the circular dichroism spectra of l-stercobilin and d-urobilin show that the conformations of these optically-active urobilinoids change with temperature between 163 and 297 degrees K. These conformational changes depend critically on the hydrogen bonding characteristics of the solvent. Thus, in methanol-glycerol (9:1), the chiral sense of the helic...

Conformation of cyclolinopeptide a observed by circular dichroism.

A stereochemical investigation, by circular dichroism, of a synthetic nonapeptide (cyclolinopeptide A) in several organic and organic-sulfuric acid solvents is presented. From this examination, and results found for a conformationally rigid model compound, 1,7,7-trimethyl-3-azabicyclo [2.2.1] heptan-2-one(camphorolactam), it is concluded that cyclolinopeptide A may exist in several conformation...

Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism.

To elucidate the basic structure of biological membranes, experimental evidence concerning the detailed molecular organization of the proteins of membranes is required. This evidence has been almost totally lacking or ambiguous up to the present time. The Cotton effects due to the peptide bond ultraviolet absorption bands, as studied by ORD and CD, have recently proved extremely valuable in cha...